Closing the gaps in kinetoplast DNA network replication

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Closing the gaps in kinetoplast DNA network replication.

T rypanosomatids are protozoan parasites responsible for important tropical diseases. One example, Trypanosoma brucei, causes African sleeping sickness, and related parasites cause Chagas disease and leishmaniasis. Because they are among the earliest-branching eukaryotes, trypanosomatids have unusual biological properties. One of their most curious features is a unique mitochondrial DNA network...

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The order Kinetoplastida is composed of several families of flagellated protozoa which are among the most ancient of the eukaryotes. The most prominent members of this order are parasites that are responsible for major tropical diseases. These parasites include Trypanosoma brucei, the African trypanosome; Trypanosoma cruzi, the South American trypanosome; and several species of Leishmania. Othe...

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The rotational dynamics of kinetoplast DNA replication.

Kinetoplast DNA (kDNA), from trypanosomatid mitochondria, is a network containing several thousand catenated minicircles that is condensed into a disk-shaped structure in vivo. kDNA synthesis involves release of individual minicircles from the network, replication of the free minicircles and reattachment of progeny at two sites on the network periphery approximately 180 degrees apart. In Crithi...

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The topology of the kinetoplast DNA network

Kinetoplast DNA (kDNA) of trypanosomatid parasites is a network of approximately 5000 catenated DNA minicircles and approximately 25 maxicircles. We developed the following strategy to deduce the topological linkage of the minicircles of the Crithidia fasciculata network. First, we used graph theory to provide precise models of possible network structures. Second, on the basis of these models, ...

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Kinetoplastid protozoa such as trypanosomes and Leishmania are important because they cause human disease. These parasites are named after one of their most unusual features, a mitochondrial DNA known as kinetoplast DNA (kDNA). Unlike all other DNA in nature, kDNA comprises a giant network of interlocked DNA rings with a topology resembling that of medieval chain mail. The replication of the kD...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 2004

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.0401400101